comet assay ii image analysis software (Perceptive Instruments Ltd)
90
Structured Review
Perceptive Instruments Ltd
comet assay ii image analysis software
Comet Assay Ii Image Analysis Software, supplied by Perceptive Instruments Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/comet+assay+ii/comet+assay+iv+software/pm40218945-303-47-53
Average 90 stars, based on 1 article reviews
Comet Assay Ii Image Analysis Software, supplied by Perceptive Instruments Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/comet+assay+ii/comet+assay+iv+software/pm40218945-303-47-53
Average 90 stars, based on 1 article reviews
comet assay ii image analysis software - by Bioz Stars,
2026-10
90/100 stars
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Staining:Article Title: Efficacy of Caffeic Acid on Diabetes and Its Complications in the Mouse Article Snippet: Afterwards, the whole blood or cell suspension of other organs was embedded in agarose (Sigma) matrix and the cells were lysed (2.5 M NaCl, 100 mM Na2EDTA, 10 mM Tris, 1% sodium sarcosinate (Sigma), 1% Triton X-100 (Sigma), 10% DMSO (Kemika), pH 10) at 4 ◦C for 1 h. Next, the slides were placed into the alkaline solution (300 mM NaOH, 1 mM Na2EDTA, pH 13) at 4 ◦C for 20 min and subsequently Molecules 2021, 26, 3262 22 of 25 electrophoresed for another 20 min at 1 V/cm. .. Finally, the slides were neutralized in 0.4 M Tris buffer (pH 7.5) for 3 × 5 min, stained with ethidium bromide (Sigma; 20 μg/mL), and analyzed at 250× magnification under an epifluorescence microscope (Zeiss, Oberkochen, Germany) connected to software ( Article Title: Radioprotective properties of food colorant sodium copper chlorophyllin on human peripheral blood cells in vitro. Article Snippet: Sodium copper chlorophyllin (CHL) is a food colorant that exhibits many beneficial properties, including potential for use in radiotherapy.. Nevertheless, genotoxicity studies investigating AC CE PT ED M AN US CR IP T Article Title: Probiotic and paraprobiotic derivates exhibit anti-inflammatory and genoprotective effects during induced stress. Article Snippet: .. The slides were stained with EtBr (Merck) and analysed using Article Title: Probiotic bacteria isolated from fermented meat displays high antioxidant and anti-inflammatory potential. Article Snippet: .. Afterwards, the slides were stained with 10 μg/ml EtBr (Merck) and a 100 randomly captured comets on two gels were analyzed using Microscopy:Article Title: Efficacy of Caffeic Acid on Diabetes and Its Complications in the Mouse Article Snippet: Afterwards, the whole blood or cell suspension of other organs was embedded in agarose (Sigma) matrix and the cells were lysed (2.5 M NaCl, 100 mM Na2EDTA, 10 mM Tris, 1% sodium sarcosinate (Sigma), 1% Triton X-100 (Sigma), 10% DMSO (Kemika), pH 10) at 4 ◦C for 1 h. Next, the slides were placed into the alkaline solution (300 mM NaOH, 1 mM Na2EDTA, pH 13) at 4 ◦C for 20 min and subsequently Molecules 2021, 26, 3262 22 of 25 electrophoresed for another 20 min at 1 V/cm. .. Finally, the slides were neutralized in 0.4 M Tris buffer (pH 7.5) for 3 × 5 min, stained with ethidium bromide (Sigma; 20 μg/mL), and analyzed at 250× magnification under an epifluorescence microscope (Zeiss, Oberkochen, Germany) connected to software ( Software:Article Title: Efficacy of Caffeic Acid on Diabetes and Its Complications in the Mouse Article Snippet: Afterwards, the whole blood or cell suspension of other organs was embedded in agarose (Sigma) matrix and the cells were lysed (2.5 M NaCl, 100 mM Na2EDTA, 10 mM Tris, 1% sodium sarcosinate (Sigma), 1% Triton X-100 (Sigma), 10% DMSO (Kemika), pH 10) at 4 ◦C for 1 h. Next, the slides were placed into the alkaline solution (300 mM NaOH, 1 mM Na2EDTA, pH 13) at 4 ◦C for 20 min and subsequently Molecules 2021, 26, 3262 22 of 25 electrophoresed for another 20 min at 1 V/cm. .. Finally, the slides were neutralized in 0.4 M Tris buffer (pH 7.5) for 3 × 5 min, stained with ethidium bromide (Sigma; 20 μg/mL), and analyzed at 250× magnification under an epifluorescence microscope (Zeiss, Oberkochen, Germany) connected to software ( Article Title: Radioprotective properties of food colorant sodium copper chlorophyllin on human peripheral blood cells in vitro. Article Snippet: Sodium copper chlorophyllin (CHL) is a food colorant that exhibits many beneficial properties, including potential for use in radiotherapy.. Nevertheless, genotoxicity studies investigating AC CE PT ED M AN US CR IP T Article Title: Comparison of DNA damage in fresh and frozen blood samples: implications for the comet assay in human biomonitoring studies. Article Snippet: The use of the comet assay in large biomonitoring studies may present logistical and technical challenges because of the processing of numerous samples.. Proper sample preservation becomes imperative to prevent spurious DNA breakage.. Previous research has shown the feasibility of conducting the comet assay on frozen blood samples, highlighting the potential of freezing at – 80 °C in preserving DNA integrity. Single Cell Gel Electrophoresis:Article Title: Efficacy of Caffeic Acid on Diabetes and Its Complications in the Mouse Article Snippet: Afterwards, the whole blood or cell suspension of other organs was embedded in agarose (Sigma) matrix and the cells were lysed (2.5 M NaCl, 100 mM Na2EDTA, 10 mM Tris, 1% sodium sarcosinate (Sigma), 1% Triton X-100 (Sigma), 10% DMSO (Kemika), pH 10) at 4 ◦C for 1 h. Next, the slides were placed into the alkaline solution (300 mM NaOH, 1 mM Na2EDTA, pH 13) at 4 ◦C for 20 min and subsequently Molecules 2021, 26, 3262 22 of 25 electrophoresed for another 20 min at 1 V/cm. .. Finally, the slides were neutralized in 0.4 M Tris buffer (pH 7.5) for 3 × 5 min, stained with ethidium bromide (Sigma; 20 μg/mL), and analyzed at 250× magnification under an epifluorescence microscope (Zeiss, Oberkochen, Germany) connected to software ( Article Title: Radioprotective properties of food colorant sodium copper chlorophyllin on human peripheral blood cells in vitro. Article Snippet: Sodium copper chlorophyllin (CHL) is a food colorant that exhibits many beneficial properties, including potential for use in radiotherapy.. Nevertheless, genotoxicity studies investigating AC CE PT ED M AN US CR IP T Article Title: Probiotic and paraprobiotic derivates exhibit anti-inflammatory and genoprotective effects during induced stress. Article Snippet: .. The slides were stained with EtBr (Merck) and analysed using Article Title: Probiotic bacteria isolated from fermented meat displays high antioxidant and anti-inflammatory potential. Article Snippet: .. Afterwards, the slides were stained with 10 μg/ml EtBr (Merck) and a 100 randomly captured comets on two gels were analyzed using Article Title: Comparison of DNA damage in fresh and frozen blood samples: implications for the comet assay in human biomonitoring studies. Article Snippet: The use of the comet assay in large biomonitoring studies may present logistical and technical challenges because of the processing of numerous samples.. Proper sample preservation becomes imperative to prevent spurious DNA breakage.. Previous research has shown the feasibility of conducting the comet assay on frozen blood samples, highlighting the potential of freezing at – 80 °C in preserving DNA integrity. other:Article Title: Characterisation and toxicological activity of three different Pseudo-nitzschia species from the northern Adriatic Sea (Croatia). Article Snippet: Diatoms of the genus Pseudo-nitzschia are cosmopolitans spread in seas and oceans worldwide, with more than 50 described species, dozens of which have been confirmed to produce domoic acid (DA).. Here, we characterized and investigated the toxicological activity of secondary metabolites excreted into the growth media of different Pseudo-nitzschia species sampled at various locations in the northern Adriatic Sea (Croatia) using human blood cells under in vitro conditions.. The results revealed that three investigated species of the genus Pseudo-nitzschia were capable of producing DA indicating their toxic potential. Article Title: Application of the comet assay for the evaluation of DNA damage from frozen human whole blood samples: Implications for human biomonitoring. Article Snippet: This study proposes the application of the comet assay for the evaluation of DNA damage from frozen human whole blood samples that could be readily used in human biomonitoring and epidemiological studies.. It was done on simply frozen whole blood samples collected from male volunteers (N= 60) aliquoted in small volumes and stored at−80 °C without the addition of cryopreservatives for a period of 5 years.. To test the applicability of the alkaline comet assay for the evaluation of DNA damage in frozen whole blood, samples were quickly thawed at 37 °C and immediately embedded in an agarose matrix followed by an alkaline comet assay procedure. Article Title: PHYSICO chemical properties and toxicological effect of landfill groundwaters and leachates Article Snippet: 19 Waste landfills represent a global problem, which is more pronounced in developing countries 20 because of the lack of resources to implement procedures that include separation and waste 21 processing.. The aim of this research was to analyze leachate and ground waters samples at the 22 site, upstream and downstream from the landfill during different year seasons on a registered 23 non-hazardous waste dump and to conduct physico-chemical and biological assays to determine 24 potential risk for the ecosystem.. Potential cytotoxic, prooxidative and mutagenic effects of 25 leachates and water samples were evaluated on human laryngeal cell line (HEp2). Migration:Article Title: Comparison of DNA damage in fresh and frozen blood samples: implications for the comet assay in human biomonitoring studies. Article Snippet: The use of the comet assay in large biomonitoring studies may present logistical and technical challenges because of the processing of numerous samples.. Proper sample preservation becomes imperative to prevent spurious DNA breakage.. Previous research has shown the feasibility of conducting the comet assay on frozen blood samples, highlighting the potential of freezing at – 80 °C in preserving DNA integrity. |